| 摘要: |
| [目的] 證實加味防己黃芪湯對于單側輸尿管梗阻（UUO）大鼠纖維化的干預作用以及對JAK2/STAT3信號通路的影響。[方法] 選取SPF級雄性SD大鼠共60只，構建UUO模型，隨機分為假手術組、模型組、中藥低劑量組、中藥中劑量組、中藥高劑量組、纈沙坦組，每組10只，術后第14天取材。觀測腎臟臟器系數、血肌酐、尿素氮、24 h尿蛋白定量；蘇木精-伊紅染色法（HE）、馬松（Masson）病理染色；Real-time qPCR法檢測NF-κB、IκBa前后表達變化；Western Blot法檢測JAK2、STAT3、p-JAK2、p-STAT3、E-鈣黏蛋白（E-cadherin）、α-平滑肌肌動特異性蛋白（α-SMA）前后表達變化。[結果] 各組大鼠腎臟臟器系數、血肌酐、尿素氮、24 h尿蛋白定量、HE、Masson病理染色結果顯示，藥物干預組較模型組明顯改善，且中劑量、高劑量組整體改善較為明顯。Real-time qPCR結果顯示，NF-κB、IκBa藥物干預組同模型組相比表達水平明顯下降（P<0.01）。Western Blot結果，E-cad、α-SMA、p-Jak2、p-Stat3蛋白表達情況，藥物干預組同模型組相比具有明顯改善（P<0.05），且中藥中劑量、高劑量組較模型組具有明顯的下調（P<0.05）。[結論] 加味防己黃芪湯能夠改善UUO大鼠的血肌酐、尿素氮、24 h尿蛋白、減輕UUO大鼠的病理損傷、下調UUO大鼠E-cad、α-SMA、p-Jak2、p-Stat3、NF-κB、IκBa的蛋白表達，從一定程度上能夠證實加味防己黃芪湯能夠干預JAK2/STAT3信號通路起到抗炎、抗纖維化的作用，對于RIF具有延緩作用。 |
| 關鍵詞: 加味防己黃芪湯 腎間質纖維化 JAK2/STAT3 UUO |
| DOI：10.11656/j.issn.1673-9043.2023.02.13 |
| 分類號:R285.5 |
| 基金項目:天津市北辰區科學技術局科技計劃項目（SHGY-2020044）；院級課題基于深度學習的智能中醫診療系統研究項目（63185021）；全國名老中醫藥專家傳承工作室建設項目：王耀光全國名老中醫藥專家傳承工作室（978022）；中醫藥事業傳承與發展項目：王耀光天津市名中醫傳承工作室（883022）。 |
|
| Intervention effect of Jiawei Fangji Huangqi Decoction on fibrosis and JAK2/STAT3 signal pathway in UUO rats |
|
HAN Yu, ZHANG Jing, ZHAO Xi, WANG Yaoguang, MA Leilei
|
|
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China
|
| Abstract: |
| [Objective] To confirm the intervention effect of modified Fangji Huangqi Decoction on fibrosis in UUO rats and the effect on JAK2/STAT3 signaling pathway.[Methods] A total of 60 SPF male SD rats were selected to establish a UUO model and randomly divided into sham operation group, model group, low-dose Chinese medicine group, medium-dose Chinese medicine group, high-dose Chinese medicine group and valsartan group, with 10 rats in each group. The samples were collected 14 d after surgery. Renal weight/body weight coefficient, serum creatinine, urea nitrogen and 24 h urinary protein were observed. HE and Masson pathological staining;the expressions of NF-κB and IκBa were investigated by Real-time qPCR. The expressions of JAK2, STAT3, p-JAK2, p-STAT3, E-cadherin and α-SMA were detected by Western Blot.[Results] The results of renal/body weight coefficient, blood creatinine, urea nitrogen, 24 h urinary protein quantification, HE and Masson pathological staining of rats in each group showed that the drug intervention group was significantly improved compared with the model group, and the overall improvement was more obvious in the medium-dose and high-dose groups. Real-time qPCR results showed that the expression levels of NF-κB and IκBa in drug intervention group were significantly decreased compared with model group(P<0.01). Western Blot results showed that the protein expressions of E-cad, α-SMA, p-Jak2 and P-Stat3 in the intervention group were significantly improved compared with the model group(P<0.05), and the medium-dose and high-dose groups were significantly down-regulated compared with the model group(P<0.05).[Conclusion] Fangji Huangqi Decoction can improve the serum creatinine, urea nitrogen and 24 h urine protein of UUO rats, reduce the pathological injury of UUO rats, and down-regulate the protein expression of E-cad, α-SMA, p-Jak2, p-Stat3, NF-κB and I-κBa of UUO rats. To a certain extent, it can be confirmed that modified Fangji Huangqi Decoction can interfere with JAK2/STAT3 signaling pathway and play an anti-inflammatory and anti-fibrosis role, which has a protective effect on RIF. |
| Key words: Jiawei Fangji Huangqi Decoction RIF JAK2/STAT3 UUO |
