| 摘要: |
| [目的] 利用快速、靈敏的UHPLC-Q-TOF-MS/MS分析技術對吳茱萸藥材的化學成分定性分析。[方法] 色譜條件為ZORBAX Eclipse plus C18色譜柱（2.1 mm×100 mm，1.8 μm），水（0.2%甲酸）-乙腈（0.2%甲酸）為流動相，流速為0.3 mL/min，柱溫30℃；采用電噴霧離子源，正負離子掃描檢測，經PeakView軟件對數據進行分析。[結果] 共鑒定出64個化合物，包括生42個生物堿、8個檸檬苦素、8個黃酮苷、3個苯丙素和3個其他類型化合物。[結論] 本實驗方案能快速、精準地對吳茱萸的化學成分進行分析，為研究吳茱萸毒性成分和藥效成分打下基礎。 |
| 關鍵詞: 吳茱萸 UHPLC-Q-TOF-MS/MS 成分分析 |
| DOI：10.11656/j.issn.1673-9043.2023.02.15 |
| 分類號:R284.1 |
| 基金項目:國家自然科學基金項目（81560648）；河北省中醫藥管理局課題項目（2018336）。 |
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| Analysis of chemical constituents from Evodia rutaecarpa by UHPLC-Q-TOF-MS/MS |
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LI Ming1, YUAN Jinbin2, ZHANG Xun3, FENG Hao3, REN Xiaojing3
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1.Tangshan People's Hospital, Tangshan 063000, China;2.Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;3.Tangshan Union Hospital, Tangshan 063000, China
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| Abstract: |
| [Objective] A rapid and sensitive UHPLC-Q-TOF-MS/MS method was used for qualitative analysis of chemical constituents of Evodia rutaecarpa.[Methods] The chromatographic conditions were ZORBAX Eclipse plus C18 column (2.1 mm×100 mm, 1.8 μm) with water (0.2% formic acid)-acetonitrile (0.2% formic acid) as mobile phase at the flow rate of 0.3 mL/min and column temperature of 30℃. Using electrospray ion source, positive and negative ion scanning detection, data in this study analysis by PeakView software.[Results] A total of 64 compounds were identified, including 42 alkaloids, 8 limonin, 8 flavone glycosides, 3 phenylpropanoids and 3 other compounds.[Conclusion] This experimental method can quickly and accurately analyze the chemical constituents of Evodia rutaecarpa, and lay a foundation for the study of the toxic components and pharmacodynamic components of Evodia rutaecarpa. |
| Key words: Evodia rutaecarpa UHPLC-Q-TOF-MS/MS component analysis |
