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| 基于HS-GC-IMS和HS-SPME-GC-MS技術對清感秋飲中揮發性成分的全面表征 |
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李巍1, 吳新龍1, 丁輝1, 尹佳鑫1, 楊文志2, 宋新波1,2,3, 李正1,2,3, 余河水1,2,3
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1.天津中醫藥大學天津中藥制藥工程學院, 天津 301617;2.組分中藥國家重點實驗室, 天津 301617;3.現代中醫藥海河實驗室, 天津 301617
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| 摘要: |
| [目的] 對清感秋飲和菊花中的揮發性成分進行全面表征。[方法] 基于頂空-氣相色譜-離子遷移譜（HS-GC-IMS）技術構建清感秋飲和菊花的揮發性成分指紋圖譜，確定共有峰；采用頂空-固相微萃取-氣質聯用（HS-SPME-GC-MS）技術對清感秋飲中揮發性成分進行定性定量分析。[結果] HS-GC-IMS方法鑒定出41種揮發性成分，HS-SPME-GC-MS方法鑒定出85種揮發性成分，其中6種揮發性成分在兩種方法中均被檢測出。此外，HS-SPME-GC-MS定量方法表明：酮類成分、烴類成分、醇類成分、酯類成分和環類成分分別占總揮發性成分的51.97％、18.15％、13.05％、6.17％和4.41％，以上幾類成分占到總揮發性成分的90％以上。[結論] 定性結合定量分析從清感秋飲中共鑒定出120種揮發性成分，其中，紫蘇屬酮、異白蘇烯酮、（Z）-乙酸菊酯、β-石竹烯等可能為其主要藥效成分。 |
| 關鍵詞: 清感秋飲 頂空-氣相色譜-離子遷移譜 頂空-固相微萃取-氣質聯用技術 揮發性成分 |
| DOI：10.11656/j.issn.1673-9043.2023.02.16 |
| 分類號:R284.1 |
| 基金項目:天津市科技計劃項目（20ZYJDJC00090）；天津市中醫藥重點領域科研項目（2021005）。 |
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| Comprehensive characterization of volatile organic compounds in Qinggan Qiuyin based on HS-GC-IMS and HS-SPME-GC-MS |
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LI Wei1, WU Xinlong1, DING Hui1, YIN Jiaxin1, YANG Wenzhi2, SONG Xinbo1,2,3, LI Zheng1,2,3, YU Heshui1,2,3
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1.College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.Medicine State Key Laboratory of Provincial and Ministerial Co-construction of Component Traditional Chinese Medicine, Tianjin 301617, China;3.Haihe Laboratory of Modern Chinese Medicine, Tianjin, 301617
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| Abstract: |
| [Objective] The volatile organic compounds(VOCs) in Qinggan Qiuyin and chrysanthemum were comprehensively analyzed.[Methods] Headspace gas chromatography-ion mobility spectrometry(HS-GC-IMS) technology was used to construct the fingerprint of VOCs of Qinggan Qiuyin and chrysanthemum, and the common peaks were identified;Headspace solid phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) technology was used for qualitative and quantitative analysis of VOCs in Qinggan Qiuyin.[Results] The 41 VOCs were identified by HS-GC-IMS, and 85 VOCs were identified by HS-SPME-GC-MS, among which 6 overlapping chemical components were detected by both methods. The quantitative results of HS-SPME-GC-MS showed that ketones, hydrocarbons, alcohols, esters and rings accounted for 51.97%, 18.15%, 13.05%, 6.17% and 4.41% of the total VOCs, and the above VOCs accounted for more than 85%.[Conclusion] A total of 120 VOCs were identified from Qinggan Qiuyin by qualitative and quantitative analysis, among which perilla ketone, isoegomaketone, (Z)-chrysanthenyl acetate, β-caryophyllene and so on may be its main pharmacodynamic components. |
| Key words: Qinggan Qiuyin headspace gas chromatography-ion mobility spectrometry headspace solid phase microextraction gas chromatography-mass spectrometry volatile organic compounds |
